ValiQuant™ HIV-1 Quantification Panel

The ValiQuant HIV-1 RNA Quantification Panel provides a standard across multiple test methods, enabling clinical laboratories and diagnostic manufacturers to assess molecular diagnostic test procedures (including the RNA extraction step) for the quantitative and qualitative determination of human immunodeficiency virus type-1 (HIV-1) RNA. The product can be used for training and proficiency testing of laboratory personnel. This product is for Research Use Only. Not for Use in Diagnostic Procedures.

Summary

The ValiQuant HIV-1 RNA Quantification Panel members were produced by making quantitative dilutions of cultured and inactivated HIV-1 Subtype B positive source material into normal human plasma (NHP).
This panel was designed and developed to meet the need for highly standardized and controlled nucleic acid testing of HIV-1 viral load. The panel helps to ensure that the nucleic acid testing procedures for HIV-1 RNA are properly validated, and allows the monitoring of training and proficiency for operators who utilize the HIV-1 RNA assay being evaluated. In order to meet global standardization and harmonization requirements, the panel has been calibrated against the World Health Organization (WHO) 2nd International Standard for HIV-1 RNA.

Principles of the Procedure

The ValiQuant HIV-1 RNA Quantification Panel members have been carefully formulated to mimic naturally occurring human specimens containing HIV-1 RNA.  Additionally, the intact virus format of the ValiQuant HIV-1 RNA Quantification Panel allows for verification of an effective viral RNA extraction procedure. As such, the test methodology must include an extraction step that releases the viral RNA and makes it available for transcription, amplification and/or detection, as appropriate to the test
This product contains 0.05% sodium azide and 0.05% gentamicin sulfate as preservatives.
The target concentrations above are manufacturing targets and may not represent actual results reported by the HIV-1 RNA quantitative assay.

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Panel Configurations

Description Catalog Number
ValiQuantTM HIV-1 RNA Quantification Panel 95-0470
Configurations Quantity
Negative 1.2mL
HIV-1 RNA 1E2 1.2mL
HIV-1 RNA 5E2 1.2mL
HIV-1 RNA 5E3 1.2mL
HIV-1 RNA 5E4 1.2mL
HIV-1 RNA 5E5 1.2mL
HIV-1 RNA 5E6 1.2mL

It is recommended that the ValiQuant HIV-1 RNA Quantification Panel be stored at -70°C or lower to ensure consistent results until the expiration date. Discard any unused material after the first use.